Keto-Test™ is a simple cow-side milk test strip that provides dairy producers an easy to use, cost-effective ketosis-screening program to help identify subclinical ketosis (SCK) in their herd.
Ketosis occurs in dairy cows as a consequence of the negative energy balance in the early postpartum period, and can result in lowered milk production, impaired fertility, and increased risk of periparturient diseases, such as displaced abomasum. Subclinical ketosis usually precedes clinical ketosis, and is much more common. Subclinical ketosis causes greater economic losses on a herd basis than clinical ketosis. Subclinical ketosis can be diagnosed by measuring ketone bodies present in blood, urine or milk. The Keto-Test™ (Ketolac test strip) is a convenient, very effective method of detecting cows with subclinical ketosis.1, 2
Beta-hydroxybutyric acid in the milk passes through the reagent part of the test strip and is convenrted by beta-hydroxybutyric acid dehydrogenase (BHBDH) to acetoacetic acid (AcAc).The NADH produced from NAD in the process reduces nitrotetrazolium blue (NTB) to fomazan, which is purple in colour. The concentration of beta-hydroxybutyric acid in the milk can then be estimated from the change in colour.